DRIP  Tutorial

February, 2019

DRIP (drug-RNA interaction predictor) is an artificial intelligence based tool that employed computational methods for identifying chemical small molecules and RNA interactions remain largely undefined. Targeting RNAs using chemical small molecules represents one fascinating strategy for disease therapeutics in the future. Therefore, we built an artificial intelligence based method called DRIP, which learned from the currently known RNA-small molecule complex structures and scored RNA-small molecule interactions according to features from both sides. DRIP would be helpful in prioritizing lead compounds targeting RNAs.
The detailed usage of the DRIP is as followings:
[1] Visiting DRIP: Log on DRIP at http://www.rnanut.net/drip/, as shown in Figure 1.

fig1
Figure 1. The homepage of DRIP

[2] Analysis of DRIP. The analysis procedure is as followings.
(1)Click the menu "Analyze" (red "1" in Figure 2).
(2) Enter RNA sequences in fasta format (red "2" in Figure2) and the structure information of chemical small molecules (red "3" in Figure2).
(3) Click the button "submit" to start analyzing (red "4" in Figure2).

fig2
Figure 2. Enter RNA sequences and the structure information of CSM

Notice:
(1) There is an "Interacting sample" (red "5" in Figure2) to show the example.
(2) The number of RNA sequences should be less than 5 and the length of RNA sequence should be smaller than 20000. The format of RNA sequence is Fasta format.
(3) The number of chemical small molecules should be less than 5. The format of structure information of chemical small molecules is the SMI format.
[3] Download the result of analysis. 
(1)Click the menu "Result" (red "1" in Figure 3).
(2)Click the result link to show the result (red "2" in Figure 3).
(3)If you have previously submitted a task, you could fill in the task ID (red "3" in Figure 3) and click the button "submit" (red "4" in Figure 3) to obtain the result. Notice that the results would be saved for half a year.

fig3
Figure 3. Download the result of analysis.